Modern Methods for Lipid AnalysiS by Liquid Chromatography/ mass spectrometry and Related Techniques

Resources for Lipid Analysis in the 21st Century

Chapter one

 

Atmospheric Pressure Ionization Techniques in

Modern Lipid Analysis

 

William Craig Byrdwell

 

Food Composition Laboratory, USDA, ARS, BHNRC, 10300 Baltimore Ave., Bldg. 161, Beltsville, MD 20705

 

Introduction

 

Some classes of lipids are large molecules that are not sufficiently volatile to allow gas-phase analysis by means such as gas chromatography (GC). Often, non-volatile lipids can be saponified to remove the fatty acid chains and these can be derivatized to yield volatile molecules, such as fatty acid methyl esters (FAME), pyrolidides, picolinyl esters, and others. These derivatives may be readily analyzed by gas chromatography in combination with a variety of detectors, including mass spectrometry. FAME analysis by GC with flame ionization detection (FID) is a common analytical method that provides valuable information regarding the net fatty acid chain composition of a sample. However, GC of FA derivatives can provide only an approximation of the composition of the original intact molecules. If there is non-random distribution of the FA chains in the parent molecules, FAME analysis can provide no indication of the distribution of those chains on the original molecules. It is preferable to analyze the large lipid molecules intact. Liquid chromatography has become the method of choice for separation of many classes of large lipid molecules. But the choice of liquid chromatography for separation inherently implies limitations in the choices available for detection. The flame ionization detector for liquid chromatography, refractive index and ultraviolet/visible (UV/vis) detectors, and more recently the evaporative light-scattering detector (ELSD) have all been employed for detection of lipids. But these two-dimensional detectors require complete chromatographic resolution to allow all molecular species to be identified. Unfortunately, even simple natural samples of lipids often contain species with the same equivalent carbon number (ECN) that overlap partially or entirely. The ECN is a measure of the overall non-polar characteristic of a fatty acid chain or molecule, given as the number of carbon atoms minus two times the number of double bonds (sites of unsaturation), ECN = Ncarb – (2 × U). For example, a 16:0 (palmitic) acyl chain has the same ECN (16 – 0 =16) as an 18:1 acyl chain (18 – 2 = 16). On reversed-phase chromatographic systems, molecules containing FA chains with the same ECN elute with similar retention times.

Contact the author:

 

Dr. William Craig Byrdwell

Food Composition Laboratory

USDA, ARS, BHNRC

10300 Baltimore Ave., Bldg. 161

Beltsville, MD  20705

 

Phone: 301-504-9357

E-mail: Byrdwell@hplc-ms.com

Web address:

http://www.byrdwell.com

This was a sample page from the book to give you an idea of what is discussed. 

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